JOHN H. LIVELY, Managing Partner

john.lively@practus.com

7001 N. Scottsdale Road, Suite 2040

Scottsdale, AZ 85253

(913) 660-0778

November 5, 2026

U.S. Securities and Exchange Commission
Filing Desk
100 F Street, N.E.
Washington, D.C. 20549

RE: Yorkville America Investment Trust (the “Trust”) (File Nos. 333-29289 and 811-08255)

Ladies and Gentlemen:

Pursuant to Rule 497(j) under the Securities Act of 1933, we hereby certify on behalf of the above registered management investment company, that the Prospectus and Statement of Additional Information that would have been filed under Rule 497(c) do not differ from that contained in the update to the Registration Statement on Form N-1A which was filed with the Commission on October 28, 2026 (Accession No. 0001040674-26-000200) for the Truth Social America First ETF, a portfolio series of the Trust.

If you have any questions concerning the foregoing, please contact the undersigned at (913) 660-0778 or John.Lively@Practus.com.

Very truly yours,

/s/ John H. Lively

On behalf of Practus, LLP