EX-FILING FEES

Calculation of Filing Fee Tables
F-3
EUROSEAS LTD.

Table 1: Newly Registered and Carry Forward Securities	☐Not Applicable

		Security Type	Security Class Title	Fee Calculation or Carry Forward Rule	Amount Registered	Proposed Maximum Offering Price Per Unit	Maximum Aggregate Offering Price	Fee Rate	Amount of Registration Fee	Carry Forward Form Type	Carry Forward File Number	Carry Forward Initial Effective Date	Filing Fee Previously Paid in Connection with Unsold Securities to be Carried Forward
Newly Registered Securities
		Equity	Common Shares, par value $0.03 per share(1)(2)(3)	457(o)
		Equity	Preferred Shares, par value $0.01 per share(2)(4)(8)(9)	457(o)
		Debt	Debt Securities(2)(5)(8)(9)	457(o)
		Other	Warrants(2)(6)(8)(9)	457(o)
		Other	Units(2)(7)(8)(9)	457(o)
Fees to be Paid	1	Unallocated (Universal) Shelf		457(o)			$ 400,000,000.00	0.0001381	$ 55,240.00
Fees Previously Paid
Carry Forward Securities
Carry Forward Securities
			Total Offering Amounts:				$ 400,000,000.00		$ 55,240.00
			Total Fees Previously Paid:						$ 0.00
			Total Fee Offsets:						$ 52,753.46
			Net Fee Due:						$ 2,486.54

Offering Note

¹	(1) Pursuant to Rule 416 under the Securities Act of 1933 (Securities Act), as amended, the common shares being registered hereunder include such indeterminate number of shares as may be issuable as a result of stock splits, stock dividends or similar transactions or as a result of the operation of anti-dilutive provisions and adjustments to conversion ratios. (2) Estimated solely for the purpose of calculating the registration fee pursuant to Rule 457(o) under the Securities Act. Pursuant to instructions on Form F-3, the table does not specify by each class information as to the proposed maximum aggregate offering price. Any securities registered hereunder may be sold separately or as units with other securities registered hereunder. In no event will the aggregate offering price of all securities sold by Euroseas Ltd. pursuant to this registration statement exceed $400,000,000. (3) Preferred stock purchase rights are not currently separable from the common stock and are not currently exercisable. The value attributable to the preferred stock purchase rights, if any, will be reflected in the market price of the common stock. (4) There is being registered hereunder an indeterminate number of preferred shares as may from time to time be sold at indeterminate prices not to exceed the aggregate offering price of $400,000,000 for all securities sold by Euroseas Ltd. pursuant to this registration statement. (5) If any debt securities are issued at an original issue discount, then the offering may be in such greater principal amount as shall result in a maximum aggregate offering price not to exceed the aggregate offering price of $400,000,000 for all securities sold by Euroseas Ltd. pursuant to this registration statement. (6) There is being registered hereunder an indeterminate number of warrants as may from time to time be sold at indeterminate prices not to exceed the aggregate offering price of $400,000,000 for all securities sold by Euroseas Ltd. pursuant to this registration statement. (7) There is being registered hereunder an indeterminate number of units as may from time to time be sold at indeterminate prices not to exceed the aggregate offering price of $400,000,000 for all securities sold by Euroseas Ltd. pursuant to this registration statement. Units may consist of any combination of the securities registered hereunder. (8) Determined in accordance with Section 6(b) of the Securities Act to be $55,240 which is equal to 0.0001381 multiplied by the proposed maximum aggregate offering price of $400,000,000 for newly registered securities.

Table 2: Fee Offset Claims and Sources	☐Not Applicable

		Registrant or Filer Name	Form or Filing Type	File Number	Initial Filing Date	Filing Date	Fee Offset Claimed	Security Type Associated with Fee Offset Claimed	Security Title Associated with Fee Offset Claimed	Unsold Securities Associated with Fee Offset Claimed	Unsold Aggregate Offering Amount Associated with Fee Offset Claimed	Fee Paid with Fee Offset Source
Rules 457(b) and 0-11(a)(2)
Fee Offset Claims
Fee Offset Sources
Rule 457(p)
Fee Offset Claims	1	Euroseas Ltd.	F-3	333-269066	12/29/2022		$ 52,753.46	Unallocated (Universal) Shelf	(1)		$ 400,000,000.00
Fee Offset Sources		Euroseas Ltd.	F-3	333-177014		09/27/2011						$ 46,440.00
Fee Offset Sources		Euroseas Ltd.	F-3	333-208305		12/02/2015						$ 0.00
Fee Offset Sources		Euroseas Ltd.	F-3	333-237128		03/12/2020						$ 8,881.69
Fee Offset Sources		Euroseas Ltd.	F-3	333-269066		12/29/2022						$ 165.77

Rule 457(p) Statement of Withdrawal, Termination, or Completion:

¹	(1) Euroseas Ltd. previously paid a registration fee of $46,648 with respect to securities that were previously registered under a registration statement on Form F-3 on September 27, 2011 (the 2011 F-3) (File No. 333-177014) that registered an aggregate of $400,000,000 of an indeterminate number of securities to be offered by Euroseas Ltd. from time to time. None of the $400,000,000 of securities registered under the 2011 F-3 were sold, and the full amount of the associated unused registration fee of $46,648 was subsequently applied to offset the registration fee payable in connection with the 2015 Registration Statement. In 2015, Euroseas filed a registration statement on Form F-3 filed on December 2, 2015 as amended on Form F-3/A filed on December 12, 2016 (File No. 333-208305) (the 2016 F-3) registering an aggregate of $400,000,000 of an indeterminate number of securities to be offered by Euroseas Ltd. from time to time. Pursuant to Rule 457(p) under the Securities Act, Euroseas Ltd. fully offset the registration fee of $41,397. In 2020, Euroseas filed a registration statement on Form F-3 filed on March 12, 2020 (File No. 333-237128) (the 2020 F-3) registering an aggregate of $400,000,000 of an indeterminate number of securities to be offered by Euroseas Ltd. from time to time. Pursuant to Rule 457(p) under the Securities Act, Euroseas Ltd. offset the registration fee of $52,588 by $43,706 representing the amount of the registration fee associated with unsold securities and paid a registration fee of $8,882. In 2022, out of the $400,000,000 securities registered in the 2020 F-3, $398,495,771 remained unsold. Euroseas Ltd. filed a registration statement on Form F-3 filed on December 29, 2022 (File No. 333-269066) (the 2022 F-3) registering an aggregate of $400,000,000 of an indeterminate number of securities to be offered by Euroseas Ltd. from time to time. In connection with the 2022 F-3, Euroseas Ltd. paid an additional $166. Of the $400,000,000 of securities registered on 2022 F-3, all of the securities remain unsold, leaving $52,753 in previously paid fees available for future offset. Pursuant to Rule 457(p) under the Securities Act, Euroseas Ltd. is offsetting the registration fee due under this registration statement by $52,753, which represents the portion of the registration fee previously paid with respect to $400,000,000 of unsold securities (the Unsold Offset Securities) previously registered on the prior registration statements. The offering of the Unsold Offset Securities pursuant to the prior registration statements associated with the claimed fee offset pursuant to Rule 457(p) has been completed or terminated. Accordingly, an additional registration fee of $2,486.54 is due to be paid at this time.

Table 3: Combined Prospectuses	☑Not Applicable

	Security Type	Security Class Title	Amount of Securities Previously Registered	Maximum Aggregate Offering Price of Securities Previously Registered	Form Type	File Number	Initial Effective Date