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Exhibit 8.1

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Subsidiaries of Galapagos NV on 31/12/2025

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Name of Subsidiary

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Jurisdiction of Incorporation or Organization

Galapagos B.V.

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The Netherlands

Galapagos U.K. Ltd

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United Kingdom

Galapagos SASU

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France

Galapagos GmbH

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Switzerland

Galapagos Real Estate Netherlands B.V.

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The Netherlands

GLPG US Holding Inc. (formerly Galapagos, Inc.)

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United States

GLPG US Inc. (formerly AboundBio, Inc.)

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United States

Xenometrix, Inc. (in liquidation)

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United States

Galapagos Holding Pte. Ltd.

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Singapore

Galapagos (Shanghai) Bioscience Co. Ltd.

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China

Galapagos Cell Therapeutics NV

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Belgium

Lakefront Biotherapeutics, Inc.

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United States

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