144 0001522780 XXXXXXXX LIVE 0001623526 STOKE THERAPEUTICS, INC. 001-38938 45 Wiggins Ave Bedford MA 01730 781-430-8200 EDWARD KAYE Former Affiliate Common Morgan Stanley Smith Barney LLC Executive Financial Services

1 New York Plaza 8th Floor New York NY 10004

1496042 38418358.56 64526242 10/21/2026 NASDAQ Common 10/21/2026 Exercise of Options Under a Registered Plan Issuer N 27441 10/21/2026 Cash Common 10/21/2026 Exercise of Options Under a Registered Plan Issuer N 15360 10/21/2026 Cash Common 10/21/2026 Exercise of Options Under a Registered Plan Issuer N 51498 10/21/2026 Cash Common 10/21/2026 Exercise of Options Under a Registered Plan Issuer N 416884 10/21/2026 Cash Common 10/21/2026 Exercise of Options Under a Registered Plan Issuer N 88817 10/21/2026 Cash Common 10/21/2026 Exercise of Options Under a Registered Plan Issuer N 9876 10/21/2026 Cash Common 10/21/2026 Exercise of Options Under a Registered Plan Issuer N 187124 10/21/2026 Cash Common 10/21/2026 Exercise of Options Under a Registered Plan Issuer N 303083 10/21/2026 Cash Common 10/21/2026 Exercise of Options Under a Registered Plan Issuer N 4917 10/21/2026 Cash Common 10/21/2026 Exercise of Options Under a Registered Plan Issuer N 67789 10/21/2026 Cash Common 10/21/2026 Exercise of Options Under a Registered Plan Issuer N 3 10/21/2026 Cash Common 10/21/2026 Exercise of Options Under a Registered Plan Issuer N 186375 10/21/2026 Cash Common 10/21/2026 Exercise of Options Under a Registered Plan Issuer N 92321 10/21/2026 Cash Common 10/21/2026 Exercise of Options Under a Registered Plan Issuer N 7054 10/21/2026 Cash Common 10/21/2026 Exercise of Options Under a Registered Plan Issuer N 37500 10/21/2026 Cash N Edward Kaye

45 Wiggins Ave Bedford MA 01730

Common 08/14/2026 12567 402521.01 This Form 144 is being filed for the cashless exercise of options and subsequent sales of the underlying commencing on 10/21/2026 10/21/2026 /s/ Edward Michael Kaye