X0202 SCHEDULE 13D/A 0001632108 XXXXXXXX LIVE 1 Common Stock, par value $0.001 per share 09/15/2026 true 0000093314 928661206 VolitionRx Limited

1489 West Warm Springs Road Suite 110 Henderson NV 89014

Jason A. Ozone (212) 309-7660 Lagoda Investment Management, L.P. 3 Columbus Circle, Floor 15 New York NY 10019 0001632108 N Lagoda Investment Management, L.P. WC OO N DE 0.00 1650505.00 0.00 1650505.00 1650505.00 N 5.9 IA See Item 2 of the Schedule 13D for additional information. Y Lagoda Investment Management, LLC OO N DE 0.00 1650505.00 0.00 1650505.00 1650505.00 N 5.9 HC See Item 2 of the Schedule 13D for additional information. Y Fatima Dickey OO N X1 0.00 1650505.00 0.00 1650505.00 1650505.00 N 5.9 IN See Item 2 of the Schedule 13D for additional information. Common Stock, par value $0.001 per share VolitionRx Limited 1489 West Warm Springs Road Suite 110 Henderson NV 89014 This Amendment No. 1 to Schedule 13D (this "Amendment") amends and supplements the Schedule 13D filed by the Reporting Persons with the Securities and Exchange Commission on September 4, 2026 (the "Schedule 13D"). Capitalized terms used but not defined in this Amendment have the meanings given to them in the Schedule 13D. Except as specifically amended by this Amendment, the Schedule 13D remains unchanged. Not amended. Not amended. Not amended. As of the date of this Amendment, each of the Reporting Persons may be deemed to beneficially own, in the aggregate, 1,650,505 shares of Common Stock, consisting of (i) 1,479,450 shares of Common Stock held in the Accounts and (ii) warrants exercisable for an aggregate of 171,055 shares of Common Stock held in the Accounts. The aggregate holdings reported herein represent approximately 5.9% of the outstanding Common Stock, calculated in accordance with Rule 13d-3 of the Securities Exchange Act based on (x) 27,903,326 shares of Common Stock outstanding as of September 14, 2026, as reported in the Issuer's Current Report on Form 8-K filed with the Securities and Exchange Commission on September 15, 2026, plus (y) 171,055 shares of Common Stock issuable upon exercise of warrants deemed beneficially owned by the Reporting Persons. See rows (7) through (10) of the cover pages to this Amendment, which are incorporated herein by reference. On September 16, 2026, the Investment Manager sold 1,500 shares of Common Stock in an open-market transaction at a price of $0.2855 per share. Except as set forth above, the Reporting Persons have not effected any other transactions in the Common Stock since September 4, 2026. Not amended. Not applicable. Not amended. Not amended. Lagoda Investment Management, L.P. /s/ Jason A. Ozone 09/16/2026 Lagoda Investment Management, LLC /s/ Fatima Dickey 09/16/2026 Fatima Dickey /s/ Fatima Dickey 09/16/2026 Not applicable.