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Notice of Proposed Sale · 144

BillionToOne, Inc.

BLLNNASDAQEQUITYCurrent

Notice of Proposed Sale

Filed Sep 24, 2026Accepted Sep 24, 2026, 5:02 PM EDTFiling CIK 2087127Accession 0001959173-26-007109
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Structured filing — 144

primary_doc.xml

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Notice of proposed sale. The proposed amounts below do not establish that a sale occurred.

Proposed-sale notice

Issuer
BillionToOne, Inc.
Issuer CIK
0002070849
SEC file number
001-42934
Phone
650-460-2551
Person for whose account securities are to be sold
Tsao David
Relationships to issuer
Officer · Director
Street
1035 O'BRIEN DRIVE
City
MENLO PARK
State / country code
CA
Postal code
94025

Securities proposed for sale

Securities proposed for sale · 1 reported row
SecurityProposed unitsAggregate market valueUnits outstandingApproximate sale dateExchangeBroker / market maker
Class A3,333416,625.0042,644,38809/24/2026NASDAQFidelity Brokerage Services LLC

Broker / market maker — proposed row 1, entry 1

Name
Fidelity Brokerage Services LLC
Street
900 Salem Street
City
Smithfield
State / country code
RI
Postal code
02917

Acquisition of securities to be sold

Acquisition of securities to be sold · 1 reported row
SecurityAcquiredNature of acquisitionAcquired fromAmount acquiredGift transactionPayment dateNature of payment
Class A09/24/2026Stock Option ExerciseIssuer3,333N09/24/2026Cash

Sales reported in the preceding three months

Sales reported in the preceding three months · 7 reported rows
SellerSecuritySale dateAmount soldGross proceeds
David TsaoClass A07/13/202620,0002,497,697.77
Dt 2021 Irrevocable TrustClass A07/13/20263,500439,110.00
David TsaoClass A08/10/202610,000928,600.00
Dt 2021 Irrevocable TrustClass A08/10/20263,500325,010.00
David TsaoClass A08/25/202610,0001,000,000.00
David TsaoClass A09/09/202620,0001,975,400.00
Dt 2021 Irrevocable TrustClass A09/09/20261,750170,695.00

Seller — prior-sale row 1, entry 1

Name
David Tsao
Street
1035 OBrien Drive
City
Menlo Park
State / country code
CA
Postal code
94025

Seller — prior-sale row 2, entry 1

Name
Dt 2021 Irrevocable Trust
Street
1035 OBrien Drive
City
Menlo Park
State / country code
CA
Postal code
94025

Seller — prior-sale row 3, entry 1

Name
David Tsao
Street
1035 OBrien Drive
City
Menlo Park
State / country code
CA
Postal code
94025

Seller — prior-sale row 4, entry 1

Name
Dt 2021 Irrevocable Trust
Street
1035 OBrien Drive
City
Menlo Park
State / country code
CA
Postal code
94025

Seller — prior-sale row 5, entry 1

Name
David Tsao
Street
1035 OBrien Drive
City
Menlo Park
State / country code
CA
Postal code
94025

Seller — prior-sale row 6, entry 1

Name
David Tsao
Street
1035 OBrien Drive,
City
Menlo Park
State / country code
CA
Postal code
94025

Seller — prior-sale row 7, entry 1

Name
Dt 2021 Irrevocable Trust
Street
1035 OBrien Drive
City
Menlo
State / country code
CA
Postal code
94025

Prior-sale reporting

Nothing to report for the preceding three months
N

Remarks

Sales within the past 3 months occurred in the account of the Dt 2021 Irrevocable Trust by Icontrust Llc, trustee on the account. David Tsao is an account stakeholder in the Dt 2021 Irrevocable Trust.

Notice signature

Notice date
09/24/2026
Signed
/s/Wade Moss, as a duly authorized representative of Fidelity Brokerage Services LLC, as attorney-in-fact for David Tsao
Plan adoption dates
06/05/2026

Company context

Current securities

Recent company filings

  1. 4 filingSep 21, 2026
  2. 144 filingSep 17, 2026
  3. 4 filingSep 10, 2026
  4. 144 filingSep 8, 2026
  5. 4 filingAug 28, 2026

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